Composite

Part:BBa_K1632033:Design

Designed by: Riku Shinohara   Group: iGEM15_Tokyo_Tech   (2015-08-30)


fim switch[default OFF](Tokyo_Tech/J23119)_rbs_gfp_rbs_lasI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 98
    Illegal NheI site found at 121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 92
    Illegal BglII site found at 1865
    Illegal BamHI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1427
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1102


Design Notes

sequence confirmed

Materials and Methods

Source

Composite of BBa_K1632003, BBa_S03154

References